Cis-Dichiorodiammineplatinum (II) (cis-DDP), a metallic compound, is a bifunctional alkylating agent that has been known as an effective anticancer drug. It is so effec-tive on solid tumor that it has been used in the therapy of ovarian tumor, epithelial tumor of the neck, bladder cancer and thyroid cancer.
Since cis-DDP reacts with DNA and RNA especially with guanine base, the pro-tein synthesis is inhibited consequently. As a result, administration of cis -DDP results in cytotoxicity of the renal epithelial cell.
So the author undertook the present study to pursue the effect of cis-DDP on the activity of acid phosphatase of the hamster kidney. Hamsters, weighing 110 gm, were used as experimental animals. The experimental animals were killed at 2, 4, and 6 days after administration of 6 mg/kg of cis-DDP. The specimens obtained from the.. kidney were sectioned at 16 μm thickness with frozen cytostat. The acid phopphatase activity was observed by the Gomori.´s method for the histochemical study.
The results were as follows.
1. The acid phospatase activity was moderate positive at the cortical glomeruli and weak positive at the outer stripes of the medulla in the 2 day cis-DDP treated group.
2. The acid phosphatase, activity was moderate positive at the pars convoluta glomeruli of thecortex and other strips of the medulla while a weak positive activity was seen at the inner stripes´ of the medulla in the 4 day cis-DDP treated group.
3. The acid phosphatase activity was moderate at -the pars convoluta and renal medulla, and strong positive activity was seen at the glomeruli in the 6 day cis-DDP treated group.
It is suggested that cis-DDP decreases the acid phosphatase activities of the hamster kidney.
|